GDNF ELISA Kits Search Results


elisa kits gdnf  (Abnova)
90
Abnova elisa kits gdnf
List of all primers used with PCR and amplification conditions.
Elisa Kits Gdnf, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa kits gdnf/product/Abnova
Average 90 stars, based on 1 article reviews
elisa kits gdnf - by Bioz Stars, 2026-04
90/100 stars
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elisa kits for glial cell line-derived neurotrophic factor (gdnf)  (Promega)
90
Promega elisa kits for glial cell line-derived neurotrophic factor (gdnf)
List of all primers used with PCR and amplification conditions.
Elisa Kits For Glial Cell Line Derived Neurotrophic Factor (Gdnf), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa kits for glial cell line-derived neurotrophic factor (gdnf)/product/Promega
Average 90 stars, based on 1 article reviews
elisa kits for glial cell line-derived neurotrophic factor (gdnf) - by Bioz Stars, 2026-04
90/100 stars
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elisa kits ngf, bdnf, nt-3 gdnf  (Promega)
90
Promega elisa kits ngf, bdnf, nt-3 gdnf
Enzyme-linked immunosorbent assay <t>(ELISA)</t> analysis of neurotrophic factor release of enteric glia (EG) cells. EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA. EG cells released NGF, BDNF and <t>GDNF</t> under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments. NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.
Elisa Kits Ngf, Bdnf, Nt 3 Gdnf, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa kits ngf, bdnf, nt-3 gdnf/product/Promega
Average 90 stars, based on 1 article reviews
elisa kits ngf, bdnf, nt-3 gdnf - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


List of all primers used with PCR and amplification conditions.

Journal: BioMed Research International

Article Title: Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

doi: 10.1155/2014/186239

Figure Lengend Snippet: List of all primers used with PCR and amplification conditions.

Article Snippet: To evaluate the NTFs, the concentrations of NGF, BDNF, and GDNF in DPC-CM, as well as BMSC-CM, were measured with ELISA kits (NGF, BDNF: Millipore Corp., California, USA; GDNF: Abnova, Taipei, Taiwan) according to the manufacturers' protocols.

Techniques: Amplification, Sequencing

DPC-CM stimulated neuron-like differentiation of PC12 cells. (a) RT-PCR analysis revealed a similar expression for the NTFs NGF, GDNF, and BDNF in DPCs on passage 4 (DPCs-P4) and passage 7 (DPCs-P7), as well as BMSCs on passage 3 (BMSCs-P3) and passage 7 (BMSCs-P7). ((b)–(i)) Neuronal differentiation of PC12 cells. After culturing in DPC-CM for 10 days, PC12 cells grew several neurites from their somas (b), though the lengths of the neurites were shorter than those for positive control groups cultured in BM supplemented with 50 ng/mL NGF (c), but were significantly longer than those for the negative control groups cultured in BM (d). After culturing for 15 days, the neurites of PC12 cells cultured in DPC-CM increased in length (e). The neurites of the positive control groups interlaced to form a net (f). By contrast, the negative control groups proliferated but bore no neurites (g). Immunofluorescent cytochemical staining demonstrated the neuronal differentiation of PC12 cells that expressed β 3-Tubulin (h) and NF-200KD (i). Scale bar = 40 μ m ((b)–(g)) and 20 μ m ((h), (i)).

Journal: BioMed Research International

Article Title: Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

doi: 10.1155/2014/186239

Figure Lengend Snippet: DPC-CM stimulated neuron-like differentiation of PC12 cells. (a) RT-PCR analysis revealed a similar expression for the NTFs NGF, GDNF, and BDNF in DPCs on passage 4 (DPCs-P4) and passage 7 (DPCs-P7), as well as BMSCs on passage 3 (BMSCs-P3) and passage 7 (BMSCs-P7). ((b)–(i)) Neuronal differentiation of PC12 cells. After culturing in DPC-CM for 10 days, PC12 cells grew several neurites from their somas (b), though the lengths of the neurites were shorter than those for positive control groups cultured in BM supplemented with 50 ng/mL NGF (c), but were significantly longer than those for the negative control groups cultured in BM (d). After culturing for 15 days, the neurites of PC12 cells cultured in DPC-CM increased in length (e). The neurites of the positive control groups interlaced to form a net (f). By contrast, the negative control groups proliferated but bore no neurites (g). Immunofluorescent cytochemical staining demonstrated the neuronal differentiation of PC12 cells that expressed β 3-Tubulin (h) and NF-200KD (i). Scale bar = 40 μ m ((b)–(g)) and 20 μ m ((h), (i)).

Article Snippet: To evaluate the NTFs, the concentrations of NGF, BDNF, and GDNF in DPC-CM, as well as BMSC-CM, were measured with ELISA kits (NGF, BDNF: Millipore Corp., California, USA; GDNF: Abnova, Taipei, Taiwan) according to the manufacturers' protocols.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control, Cell Culture, Negative Control, Staining

DPCs promoted neurite outgrowth in PC12 cells and secreted BDNF and GDNF. ((a)–(h)) After culturing for 4 days, the average neurite lengths of PC12 cells cultured in DPC-CM supplemented with various concentrations of NGF ((a)–(d)) were longer than those of cells cultured in BM supplemented with the same concentrations of NGF ((e)–(h)), as indicated by phase contrast microscopic observation. (i) The PC12 cells cultured in DPC-CM supplemented with 10 ng/mL NGF displayed the longest average neurite lengths compared with other concentrations of NGF (0 ng/mL, 5 ng/mL, and 30 ng/mL), but there was no statistical difference with high dose NGF (30 ng/mL) ( P > 0.05). ((j), (k)) The amount of BDNF (j) and GDNF (k) secreted per 10 thousand cells in 5 mL DPC-CM, BMSC-CM, and BM for 24 h. Scale bar = 20 μ m ((a)–(h)). * P < 0.05. ** P < 0.01.

Journal: BioMed Research International

Article Title: Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

doi: 10.1155/2014/186239

Figure Lengend Snippet: DPCs promoted neurite outgrowth in PC12 cells and secreted BDNF and GDNF. ((a)–(h)) After culturing for 4 days, the average neurite lengths of PC12 cells cultured in DPC-CM supplemented with various concentrations of NGF ((a)–(d)) were longer than those of cells cultured in BM supplemented with the same concentrations of NGF ((e)–(h)), as indicated by phase contrast microscopic observation. (i) The PC12 cells cultured in DPC-CM supplemented with 10 ng/mL NGF displayed the longest average neurite lengths compared with other concentrations of NGF (0 ng/mL, 5 ng/mL, and 30 ng/mL), but there was no statistical difference with high dose NGF (30 ng/mL) ( P > 0.05). ((j), (k)) The amount of BDNF (j) and GDNF (k) secreted per 10 thousand cells in 5 mL DPC-CM, BMSC-CM, and BM for 24 h. Scale bar = 20 μ m ((a)–(h)). * P < 0.05. ** P < 0.01.

Article Snippet: To evaluate the NTFs, the concentrations of NGF, BDNF, and GDNF in DPC-CM, as well as BMSC-CM, were measured with ELISA kits (NGF, BDNF: Millipore Corp., California, USA; GDNF: Abnova, Taipei, Taiwan) according to the manufacturers' protocols.

Techniques: Cell Culture

Isolation and characterization of NCSCs-like cells. (a) After suspension in culture for 7 days, neurosphere-like spheres were generated from DPCs (black arrow), along with non-sphere-forming DPCs (white arrow). ((b)–(e)) Coexpression of Nestin (b) and P75 (c) in spheres were detected by double immunostaining. Nuclei were stained with Hoechst 33342 (d). The images were merged (e). (f) RT-PCR showed a higher expression level of NCSCs-specific markers in sphere-forming DPCs compared with non-sphere-forming DPCs and total DPCs on the same passage. (g) RT-PCR revealed similar expression levels of NTFs in sphere-forming DPCs, non-sphere-forming DPCs, and total DPCs. ((h), (i)) The amount of BDNF (h) and GDNF (i) secreted per 10 thousand sphere-forming DPCs and total DPCs in 5 mL supernatant for 24 h. Scale bar = 20 μ m ((a), (b)–(e)). * P < 0.05.

Journal: BioMed Research International

Article Title: Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

doi: 10.1155/2014/186239

Figure Lengend Snippet: Isolation and characterization of NCSCs-like cells. (a) After suspension in culture for 7 days, neurosphere-like spheres were generated from DPCs (black arrow), along with non-sphere-forming DPCs (white arrow). ((b)–(e)) Coexpression of Nestin (b) and P75 (c) in spheres were detected by double immunostaining. Nuclei were stained with Hoechst 33342 (d). The images were merged (e). (f) RT-PCR showed a higher expression level of NCSCs-specific markers in sphere-forming DPCs compared with non-sphere-forming DPCs and total DPCs on the same passage. (g) RT-PCR revealed similar expression levels of NTFs in sphere-forming DPCs, non-sphere-forming DPCs, and total DPCs. ((h), (i)) The amount of BDNF (h) and GDNF (i) secreted per 10 thousand sphere-forming DPCs and total DPCs in 5 mL supernatant for 24 h. Scale bar = 20 μ m ((a), (b)–(e)). * P < 0.05.

Article Snippet: To evaluate the NTFs, the concentrations of NGF, BDNF, and GDNF in DPC-CM, as well as BMSC-CM, were measured with ELISA kits (NGF, BDNF: Millipore Corp., California, USA; GDNF: Abnova, Taipei, Taiwan) according to the manufacturers' protocols.

Techniques: Isolation, Suspension, Generated, Double Immunostaining, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

Enzyme-linked immunosorbent assay (ELISA) analysis of neurotrophic factor release of enteric glia (EG) cells. EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA. EG cells released NGF, BDNF and GDNF under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments. NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.

Journal: Neural Regeneration Research

Article Title: Enteric glia mediate neuronal outgrowth through release of neurotrophic factors

doi: 10.3969/j.issn.1673-5374.2012.028.001

Figure Lengend Snippet: Enzyme-linked immunosorbent assay (ELISA) analysis of neurotrophic factor release of enteric glia (EG) cells. EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA. EG cells released NGF, BDNF and GDNF under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments. NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.

Article Snippet: Subsequently, the cells were centrifuged and the growth factors were measured in the supernatant using commercially available ELISA kits for NGF, BDNF, NT-3 and GDNF (Promega, Madison, WI, USA) using the protocols described by the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay, Derivative Assay